Fig. 1

Impact of CYP1A knockdown on FICZ-mediated effects on mortality and phenotype. Groups of embryos, non-injected (NI) or injected with Ctrl-MO (Ctrl) or CYP1A-MO (CYP), were exposed to vehicle control (DMSO; D), or 10 or 100 nM FICZ (F10, F100). All endpoints were determined at 3 dpt (4 dpf); (A) cumulative mortality, (B) hatching rates, (C) swim bladder inflation and (E) rates of pericardial edema and circulatory failure. Severity of pericardial edema and circulatory failure was graded 0-3, “0” representing no effect and “1-3” representing effects of increasing severity. The severity scoring index for pericardial edema is shown in (D). In (B), (C) and (E) the hatched part of a bar indicates the mortality rate. Results are shown as% of total number of embryos at exposure start based on three separate experiments (n = 69-99). Statistically significant differences in numbers of affected and unaffected embryos between the MO injected groups (Ctrl or CYP) and the corresponding NI group were determined by Fisher’s exact test with Bonferroni’s correction and are shown by asterisks (**p < 0.01 and ***p < 0.001). For the statistical analysis of data in (E) “affected embryos” are represented by the pooled number of all living embryos with severity score 1-3 in a group.