Fig. 2

Results of the multiview reconstruction. (A) Overlaid registered views, each in a different color to demonstrate the overlap between them. (B) Magnified view showing the overlap of the PSFs of beads imaged from the different views. (C) Close up of a bead after fusion, the PSF is an average of the different views. (D) PSF of the same bead as in (C) after multiview deconvolution showing that the PSF collapses into a single point. (E) x-y section and (F) y-z section of a single view of an optic vesicle, in which membranes are labeled with GFP showing degradation of the signal in z deeper in the tissue. (G) x-y section and (H) y-z section of the same view after weighted-average fusion of 4 views approximately 20 degrees apart with slightly more degraded x-y resolution overall but increased z-resolution. (I) x-y section and (J) y-z section of the same data after multiview deconvolution, showing a significant increase in resolution and contrast of the signal both in xy and in z. Images in (E-J) are single optical slices. Scale bar represents 50 µm (A, B, E-J) and 10 µm (C, D).