ZFIN ID: ZDB-FIG-160511-9
DeRossi et al., 2016 - trappc11 is required for protein glycosylation in zebrafish and humans. Molecular biology of the cell   27(8):1220-34 Full text @ Mol. Biol. Cell
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 5

Fig. 3

Tm phenocopies trappc11 mutants and synergizes with trappc11 mutation. (A) Transgenic WT and trappc11 mutant larvae that express a hepatocyte-specific marker (Tg(fabp10:dsRed)) were exposed to sublethal doses of Tm at 3 dpf and analyzed at 5 dpf for gross morphology. (B) WT, trappc11 mutants, and WT exposed to 0.5 µg/ml BFA were treated with increasing concentrations of Tm (1, 2, or 3 µg/ml) beginning at 3 dpf and scored for survival at 4 and 5 dpf (three clutches for each Tm concentration). WT, trappc11 mutants, and BFA-treated samples are respectively represented by black, blue, and green lines, with 1, 2, and 3 µg/ml Tm indicated by solid, dashed, and dotted lines, respectively. (C) Cumulative survival at 5 dpf of WT, trappc11 mutants, and WT + 0.5 µg/ml BFA exposed to Tm (1, 2, and 3 µg/ml) between 3 and 5 dpf. Black, blue, and green bars indicate WT, trappc11, and WT + BFA, respectively, with increased shading representing different clutches. Three clutches total were scored for survival. Gray segmented lines mark 100, 200, and 300% levels. (D) Livers from 5-dpf WT and trappc11 mutants treated with DMSO or 0.125 µg/ml Tm were collected and subjected to qPCR analysis for UPR-responsive genes, and PC1 metrics were calculated. Values for each individual sample. (E) Livers from 5-dpf WT and trappc11 mutants treated with DMSO, 0.25 µM thapsigargin, or 0.5 µM thapsigargin were collected and subjected to qPCR analysis for UPR-responsive genes, and PC1 metrics were calculated. The p values in C and D were calculated using Student’s t test. n.s., not significant.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU chemical treatment: tunicamycin Day 5 response to endoplasmic reticulum stress increased occurrence, abnormal
AB/TU chemical treatment: tunicamycin, chemical treatment: brefeldin A Day 5 whole organism dead, abnormal
Day 5 whole organism decreased life span, abnormal
trappc11hi1532bTg/hi1532bTg chemical treatment: tunicamycin Day 5 response to endoplasmic reticulum stress increased occurrence, abnormal
Day 5 whole organism dead, abnormal
Day 5 whole organism decreased life span, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Biol. Cell