Fig. 2

COLA response in zebrafish and C. elegans. (A and B) Illustration of the analysis of the cortical response to COLA. (A) The time evolution of the cut boundary opening was characterized by fitting an ellipse to the cut boundary and measuring the minor radius of the fitted ellipse (white solid line). To analyze the regrowth of the cortical NMY-2 after the cut, we measured the average fluorescence intensity within the box (white broken line). The size of the box is 1.8 × 1.4 µm for zebrafish and 1.4 × 1.2 µm for C. elegans. (B) To obtain the velocity profile along the direction perpendicular to the cut line (red line), the x components of the velocity vectors inside the rectangular area surrounded by the orange broken line were averaged in the y direction (box size, zebrafish: 27 × 13 µm, C. elegans: 12 × 10 µm). (C and F) Velocity profile along the direction perpendicular to the cut line at the time just after the cut for zebrafish (C) and C. elegans (F). (D and G) Time evolution of the minor radius of the fitted ellipse to the cut boundary for zebrafish (D) and C. elegans (G). (E and H) Average concentration of cortical NMY-2 as a function of time after the cut opening for zebrafish (E) and C. elegans (H). (Red) Experimental results in zebrafish (C-E) and C. elegans (F-H). Error bars represents standard error, with n = 15 for all cases. (Blue lines) Theory curves utilizing the obtained least square fit parameters, see main text.