FIGURE

Fig. 2

ID

ZDB-FIG-160414-8

Publication

Don et al., 2016 - Genetic basis of hindlimb loss in a naturally occurring vertebrate model

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Fig. 2

Mutations in the Tbx4 NLS sequence cause the loss of pelvic fins in pelvic finless zebrafish. (A) Wild-type zebrafish (tbx4^+/+) pelvic fins are located on the posterior ventral flank of the fish, either side of the cloaca. (B) TALENs-induced mutated tbx4 causes the loss of pelvic fins in homozygous tbx4 mutation zebrafish (tbx4^gi1/gi1). (C) Heterozygous mutant tbx4 zebrafish (tbx4^gi1/+) display normal pelvic fin development. (D) Pelvic finless zebrafish (pfl^mq6/mq6) do not develop pelvic fins due to missense mutations in the Tbx4 NLS. (E) Pelvic finless; mutant tbx4 compound heterozygotes (tbx4^gi1/mq6) also do not develop pelvic fins. (F) Heterozygous wild-type; pelvic finless zebrafish (pfl^mq6/+) develop pelvic fins, confirming that the pelvic finless mutation is allelic to tbx4 and that the mutations in the NLS of Tbx4 (A78V; G79A) result in a loss of function of the tbx4 gene. White rectangle in A-F indicates zoomed in region shown in A′-F′. Scale bars: 1mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx4^gi1/gi1 tbx4^mq6/mq6 tbx4^gi1/+ tbx4^mq6/+ tbx4^gi1/+; tbx4^mq6/+
Observed In:	forelimb morphogenesis hindlimb morphogenesis pectoral fin pectoral fin morphogenesis pelvic fin pelvic fin morphogenesis whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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