FIGURE

Fig. 4

ID

ZDB-FIG-160323-20

Publication

Kawasaki et al., 2016 - Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture

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Fig. 4

Differentiation of SSCs to sperm in culture. (A) SSCs that underwent propagation culture for 31days were transferred onto Sertoli ZtA6-12 feeders and cultured under the differentiation condition. After 1day of transfer, SSCs attached tightly on Sertoli feeders. Spermatocytes (inset) and sperm (inset) appeared around 10 and 17days after the transfer, respectively. Top panels: phase contrast; bottom panels: fluorescence. (B) Sycp3 expression in cells under differentiation culture. SSCs that underwent propagation culture for 31days were transferred onto ZtA6-12 feeders and cultured for 9days. Sycp3 signal is observed as a small particle on one side of the nucleus and extends into the nucleus (inset), suggesting normal meiotic initiation. (C) Sycp3 immunostaining of SSC clumps cultured under SSC propagation conditions for 40 days. Cells expressing Sycp3 were observed only rarely, at the periphery of SSC clumps (arrows). Scale bars: 50µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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