FIGURE

Fig. 4

ID

ZDB-FIG-160309-12

Publication

Tang et al., 2016 - Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish

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Fig. 4

Visualizing the emergence of clonal dominance in Myc-induced T-ALL.

(a) Donor animals engrafted with monoclonal T-ALL arising in the CG1 background. (b,c) Monoclonal T-ALLs were implanted into the syngeneic CG1 strain fish and assessed for LPC frequency by limiting dilution cell transplantation (b) or latency of regrowth (c). P-values are noted within each panel. Not significant (NS). (d,e) Confocal imaging of engrafted rag2^E450fs (casper) fish at 10 d.p.t. (d) and 23 d.p.t. (e). White arrow denotes site of injection, and the location for confocal imaging. (f) Relative proportions of each fluorescent clone contained within leukaemias from individual engrafted animals (n=16 animals). Imaging was completed on the same animals at 10-13 d.p.t. and 22-24 d.p.t. The ZsYellow+ clone dominates leukaemia regrowth in animals #1-3, whereas mCherry+ dominates in animals #13-16. Scale bars equal 5 mm in whole animal images and 50 µm in confocal images.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; rag2^fb101/fb101; mpv17^a9/a9
Condition:	cancer xenotransplantation
Observed In:	whole organism whole organism Lymphoid neoplasms
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.