FIGURE

Fig. 3

ID

ZDB-FIG-160308-4

Publication

Shen et al., 2016 - The Rag-Ragulator Complex Regulates Lysosome Function and Phagocytic Flux in Microglia

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Fig. 3

Dysregulated Lysosomal Activity in Microglia of rraga^st77/st77 Mutants

(A and B) Analysis of RNA sequencing data revealed that genes associated with lysosomal activity are significantly upregulated in rraga^st77/st77 mutants at 5 dpf. Using the DAVID Bioinformatics Resources, genes showing at least a 1.5-fold upregulation were classified according to (A) Kegg Pathway terms and (B) molecular functions (GOTERM_MF_FAT).

(C and D) Visualization of Lysotracker Red and Tg(mpeg1:EGFP) in WT (C, n = 4) and rraga^st77/st77 mutants (D, n = 4) reveals that lysosomes are increased in microglia in the mutants. Larvae shown in (C) and (D) were genotyped by PCR after photography.

Expression Data

Gene:	EGFP
Fish:	gl22Tg rraga^st77/st77; gl22Tg
Anatomical Term:	brain microglial cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rraga^st77/st77; gl22Tg
Observed In:	microglial cell lysosome microglial cell lysosome organization
Stage:	Day 4

Phenotype Detail

Acknowledgments

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