FIGURE

Fig. 1

ID

ZDB-FIG-160303-41

Publication

Merrick Schill et al., 2016 - Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse

Other Figures

Fig. 1

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Fig. 1

Ibuprofen reduced ENCDC colonization of zebrafish bowel. (A–D) Zebrafish were treated with vehicle (1% DMSO) or ibuprofen (Ibu) from 34 to 96 hpf and then stained with HuC/D antibody. (A) White arrows indicate dorsal root ganglia. (C) White arrows highlight enteric neurons. Scale bar=500 µm. (B and D) Higher magnification of fish midsection. Scale bar=100 µm. (E) Length of bowel from most distal HuC/D+ cell (arrows in B and D) to bowel terminus (arrowheads in B and D). *P<0.05 (ANOVA on Ranks) (F) Length of whole zebrafish (1% DMSO n=4, 25 µM Ibuprofen n=6). *P=0.025 (t-test). (G) Distance between DRG and dorsal zebrafish edge (1% DMSO n=3, 25 µM ibuprofen n=4). *P>0.05 (t-test).

Expression Data

Antibody:	Ab1-elavl
Fish:	AB
Condition:	chemical treatment: ibuprofen
Anatomical Terms:	dorsal root ganglion enteric neuron
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	chemical treatment: ibuprofen
Observed In:	dorsal root ganglion neural crest cell migration involved in autonomic nervous system development enteric nervous system development enteric neuron neural crest cell migration involved in autonomic nervous system development whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 409(2), Merrick Schill, E., Lake, J.I., Tusheva, O.A., Nagy, N., Bery, S.K., Foster, L., Avetisyan, M., Johnson, S.L., Stenson, W.F., Goldstein, A.M., Heuckeroth, R.O., Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse, 473-88, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.