FIGURE

Fig. S12

ID

ZDB-FIG-160303-34

Publication

Chen et al., 2016 - Efficient extravasation of tumor-repopulating cells depends on cell deformability

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Fig. S12

TRCs extravasate more effectively than control melanoma cells in vivo after co-injection. (a) TRCs were transfected with pEYFP-N1 and Cont were transfected with pECFP-N1 respectively, then mixed and co-injected at 1:1 ratio into the cavity of pericardium of 48 hpf embryos. Images in the first two panels show penetration area of Cont (left panels) or TRCs (right panels) at 1, 6, 12, and 24 hpi respectively, and the next two panels show images of vessels and 3-color overlays respectively. Dashed red lines mark the tumor extravasation areas from vessels to surrounding tissues. Scale bars, 100 µm. Total tumor cell volumes were quantitatively similar at 1, 6, and 12 hpi for Cont and TRCs, suggesting that the cell numbers were similar. (b) Quantification of extravasated tumor area relative to the total tumor area at different time points: 1, 6, 12, and 24 hpi. Color code: Zebrafish blood vessels are red, Cont are cyan, and TRCs are yellow. Mean± s.e.m.; n>6 fish per group; ≥3 independent experiments. *p< 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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