Fig. 6

Box shows location of missense mutation in IQCK; (A) control morpholino phenotype at 48 h in zebrafish is normal; (B) iqck morpholino at 48 h shows cardiac edema (red arrow) and ventral fin hypoplasia (yellow arrow); (C) coinjection of iqck morpholino and IQCK (hIQCK) mRNA shows rescued phenotype; (D) control human IQCK injection results in normal phenotype; (E) coinjection of iqck morpholino and human Q223E human IQCK mRNA resulting in worsening of phenotype than iqck morpholino alone shown in Figure 6B; (F) human Q223E IQCK mRNA injection shows normal phenotype; (G) coinjection of iqck morpholino and human Q223H human IQCK mRNA resulting failure to rescue phenotype; (H) human Q223H IQCK mRNA injection shows normal phenotype; (I) coinjection of iqck morpholino and human Q222S human IQCK mRNA resulting in a rescued phenotype; (J) human Q222S IQCK mRNA injection shows normal phenotype.