FIGURE

Fig. S1

ID

ZDB-FIG-151112-5

Publication

Sapp et al., 2014 - Fructose leads to hepatic steatosis in zebrafish that is reversed by mTOR inhibition

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Increased liver size in larvae treated with fructose. Larvae treated with 4% glucose or 4% fructose were fixed in 4% paraformaldehyde at 7 dpf. After bleaching, the larvae were subjected to in situ hybridization in accordance with standard protocols, using cp (ceruloplasmin) as riboprobe, as in previous studies (1). Three separate sets of larvae underwent in situ hybridization. (A) Average liver size, as determined by ImageJ analysis of the area of liver staining. The graph depicts the mean, normalized to glucose, with S.E.M. As mentioned, three separate trials are averaged, comprising 20, 17 and 12 larvae for glucose, and 21, 15, and 27 larvae for fructose. (B) Six representative larvae from glucose and fructose categories, with cp liver staining noted (arrow). *p≤0.05 by Student’s t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL
Condition:	chemical treatment by environment: fructose
Observed In:	liver
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hepatology