FIGURE

Fig. S4

ID
ZDB-FIG-151012-5
Publication
Swinburne et al., 2015 - Improved Long-Term Imaging of Embryos with Genetically Encoded α-Bungarotoxin
Other Figures
All Figure Page
Back to All Figure Page
Fig. S4

α-bungarotoxin protein injected into the yolk is distributed throughout the embryo.

(A) Two injection strategies explored for α-bungarotoxin protein injection: either into the ventral side of the yolk or the dorsal side of the yolk of zebrafish embryos at 24 hpf. (B) Fluorescence from 2.3 ng Alexa-Fluor 594 conjugated α-bungarotoxin injected into the ventral yolk imaged by laser-scanning confocal microscopy. The peripheral yolk space appears continuous with the fluid entering the heart. DIC images (left) and fluorescent images (right) of representative embryos receiving ventral yolk injections of 2.3 ng 3 kDa dextran-Texas red (C-D), ventral yolk injections of 2.3 ng Alexa-Fluor 594 conjugated α-bungarotoxin (E-F), dorsal yolk injections of 2.3 ng 3 kDa dextran-Texas red (G-H), and dorsal yolk injections of 2.3 ng Alexa-Fluor 594 conjugated α-bungarotoxin (I-J). Scale bar in (C) applies for (C-J).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One