Fig. 1

RGC-specific Gal4 expression in the adult zebrafish brain. a–d: Expression of GFP and GFAP in the adult Tg(gfap:Gal4FF;UAS:GFP) telencephalon. Sections of the telencephalon of Tg fish were stained with anti-GFAP (red) antibodies. Nuclei were stained with (Hoechst blue). a′–d′: Magnified images of the white-boxed areas above. e-h: Expression of GFP and BLBP in the adult Tg(gfap:Gal4FF;UAS:GFP) telencephalon. Sections of the telencephalon of Tg fish were stained with anti-BLBP (red) antibody. Nuclei were stained with Hoechst (blue). e′–h′: Magnified images of the white-boxed areas above. i: Colocalization of GFP with BLBP in the adult telencephalon. The percentage of GFP(+)/BLBP(+) cells is 97.51 ± 2.49% in the Dm domain, 98.11 ± 1.89% in the Dl domain, 98.85 ± 1.15% in the Vd domain, and 94.31 ± 5.69% in the Vv domain. Data are expressed as mean ± SEM; n = 3. Scale bars, 200 µm in a and e, 20 µm in a′ and e′.