FIGURE

Fig. S3

ID

ZDB-FIG-150921-19

Publication

Wei et al., 2015 - DNA damage during the G0/G1 phase triggers RNA-templated, Cockayne syndrome B-dependent homologous recombination

Other Figures

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Fig. S3

Cell cycle-independent and CSB-dependent recruitment of the HR factors at TA-KR damage sites. (A) U2OS TRE cells were synchronized at the G1 phase by double thymidine block and stained with PI. The graph shows distribution of the cell population by FACS. (B) TA-KR transfected U2OS TRE cells with/without light irradiation 24 h postincubation. Diameter of TA-KR was assessed by confocal microscopy. (C–E) Western blot of GFP-RAD52 and CSB (C), cell cycle profiles (D), and recruitment of YFP-POLII (E) in U2OS TRE cells pretreated with the RNA POLII inhibitor α-amanitin (100 µg/mL) for 1 h, DRB (20 µM) for 24 h, or siCSB. D shows FACS analysis of U2OS TRE cells with the same treatment as in C. (F) Western blot of GFP-RAD52 in cells with or without RNaseH treatment (15 units) for 15 min.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA