FIGURE

Fig. S6

ID

ZDB-FIG-150716-5

Publication

Kotani et al., 2015 - Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S6

Phenotypic analysis of the embryos injected with three crRNAs, tracrRNA and Cas9 protein.

(A) An uninjected embryo. (B) Tg(cmlc2:EGFP)-derived embryos injected with tracrRNA (100 pg), spns2-crRNA2 (25 pg), s1pr2-crRNA1 (25 pg) s1pr2-crRNA2 (25 pg) and Cas9 protein (400 pg). (C) Tg(cmlc2:EGFP)-derived embryos injected with spns2-gRNA1 (25 pg), s1pr2-gRNA1 (25 pg), s1pr2-gRNA2 (25 pg) and Cas9 protein (400 pg). Cardia bifida was observed in the embryo injected with multiple crRNAs, tracrRNA and Cas9 protein, whereas a normal single heart was observed in an uninjected embryo and the embryo injected with multiple gRNAs and Cas9 protein. (A-C) Ventral view with anterior at the top. White arrowheads indicate the position of the developing heart.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One