Fig. 1

Gal4 enhancer trap constructs and imaging. A: Enhancer trap constructs used to generate all lines. B1-B Max: To convey as much information as possible, compound microscopic images were taken at multiple depths (B1–B4) and used to generate Maximum Intensity Projections (B Max) in which signal from multiple focal depths is visible. Arrowheads mark the olfactory placode (blue), spinal neurons (green), otic vesicle and retina (orange) and epithelial cells (yellow). Images B1–B Max are lateral views. C–F: Confocal images enhance fine details relative to compound microscopic images. Glial endfeet are clearly visible in the confocal image (D, Arrowhead) but are not clear in the compound scope image (C, Arrowhead). Habenulae are visible in confocal (F, Orange arrowheads) but not compound (E, Orange arrowheads) image. (E,F) Pineal gland marked by yellow arrowhead. Images C–F are dorsolateral views. Scale bars = 250 µm in B1–B MAX; 250 µm in C,D; 100 µm in E,F.