Fig. 6

ptf1a lineage analysis in homozygous ptf1a^sa126/sa126 fish. In all panels, ptf1a lineage–labeled cells are shown in red. A: In wildtype zebrafish, ptf1a lineage labeling can be observed in the hindbrain and pancreas (A′). A′′ is a high-magnification, 3D-reconstructed view of the boxed region in A′. 2F11 (purple) labels gall bladder, liver, pancreatic PNCs, and some structures in the gut bulb. B: In ptf1a^sa126/sa126 fish, there is no exocrine pancreatic structure. Some lineage-labeled cells can be observed in the wall of gall bladder, as well as in another unidentified population adjacent to the gall bladder. B′′ is a zoomed-in view of the boxed region in B′. 2F11, purple. C,D: A mixture of antibodies against Insulin (Ins), Glucagon (Gluc), and Somatostatin (Sst) (green) labels the endocrine cell population. Nkx6.1 (purple) labels PNCs. C: In the wildtype zebrafish pancreas, the majority of lineage labeled cells are acinar cells. D: In homozygous ptf1a mutant fish, the number of PNCs is reduced and they appear at an ectopic location. ptf1a lineage–labeled cells can be detected in the principal islet and some of the PNCs. gb, gall bladder. lv, liver.