Fig. 4

Rossi et al., 2015 - The SLC7A7 Transporter Identifies Microglial Precursors prior to Entry into the Brain
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Slc7a7+ Cells Give Rise to Microglia in the Brain

(A–C) Lateral view shows a representative slc7a7::Kaede;fms::nfsB-mCherry embryo at 2 days post fertilization; slc7a7+ cells (A, green, slc7a7::Kaede), macrophages (B, red, fms::nfsB-mCherry), and overlay (C) are shown. Few cells are both fms+ and slc7a7+ (yellow arrowheads). White arrowheads indicate fms+ cells only.

(D–F) Frontal view shows a representative slc7a7::Kaede;pU.1::TagRFP embryo at 2 days post fertilization. Slc7a7+ cells (D, green, slc7a7::Kaede), leukocytes (E, red, pU.1::TagRFP), and overlay (F) are shown. Few cells are both pU.1+ and slc7a7+ (yellow arrowheads). White arrowheads indicate pU.1+ cells only.

(G–I) Dorsal view shows a slc7a7::Kaede;pU.1::TagRFP embryo at 4 days post fertilization. Microglia are both slc7a7+(G) and pU.1+ (H), with overlay (I) and a representative microglia (inset) shown.

(J–O) Dorsal view shows a 4-day-post-fertilization slc7a7::Kaede embryo not photoconverted (J–L) and photoconverted at 24 hpf (M–O) (Kaede green, J and M; Kaede red, K and N; overlay, L and O).

(P–R) Representative example of a photoconverted microglia is shown.

Scale bars for all images, 25 µm. See also Figure S4.

Expression Data
Anatomical Terms:
Stage Range: Long-pec to Day 4

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.