aanat2 Is Not Required for Behavioral or Molecular Circadian Rhythms (A) Larvae raised in LD and monitored in DD maintain locomotor activity circadian rhythms. Locomotor activity data (dashed lines) were fit to damped cosine curves (solid lines) to quantify circadian amplitude (B), period length (C), and phase (D). aanat2−/− larvae have reduced amplitude due to increased locomotor activity during subjective night, but period length and phase are indistinguishable from sibling controls. Black and hatched boxes indicate subjective night and day, respectively. (E–H) Tg(per3:luc) larvae were raised in LD and luminescence recorded during 100 hr in DD. (E) Dashed and solid lines indicate luminescence data and damped cosine curve fits, respectively. (F–H) Circadian rhythm amplitude, period length, and phase are not significantly different for any genotype. Bar graphs represent mean ± SEM. a.u., arbitrary units. n, number of larvae. p < 0.01 compared to aanat2−/− by Dunnett’s test. See also Figure S4.
|
