FIGURE

Fig. 3

ID

ZDB-FIG-150506-7

Publication

Ablain et al., 2015 - A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish

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Fig. 3

Tissue-Specific urod Inactivation using the CRISPR Vector

(A) Confocal images of 30 hpf embryos injected with Tol2 mRNA and the indicated vectors. The fluorescent blood phenotype associated with urod knockout is seen when using the ubiquitous ubi promoter or the erythrocyte-specific gata1 promoter with the gRNA targeting urod, but not when using the muscle-specific mylz2 promoter or a gRNA against p53. See also Figure S3A and Movie S1.

(B) Most frequent (>1%) mutant urod alleles found by deep-sequencing in sorted fluorescent red cells from the blood of embryos injected with the pcmlc2:GFP, U6:gRNA urod, gata1:Cas9 vector. The CRISPR target sequence is in green and mutations in red. The type of mutation and the associated frequency (in % of all mutated alleles) are indicated.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 32(6), Ablain, J., Durand, E.M., Yang, S., Zhou, Y., Zon, L.I., A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish, 756-64, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell