FIGURE

Fig. 4

ID

ZDB-FIG-150506-17

Publication

Campbell et al., 2015 - Dynamic visualization of transcription and RNA subcellular localization in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

MS2-tagged nos3′utr colocalizes with endogenous nanos3 and a subset of Vasa-positive germ granules in PGCs. (A,B) Endogenous nanos3 RNA localizes to PGCs at 30hpf and is present throughout the cytoplasm and in larger perinuclear accumulations (arrows). Scale bar: 10µm. (C-F) Cherry-positive cells at 30hpf have diffuse cytoplasmic MCP-GFP signal and larger perinuclear accumulations (arrows). Scale bar: 10µm. (G-J) Accumulations of MCP-GFP in Cherry-expressing PGCs at 30hpf in cherry-24xMBS-nos3′UTR-injected embryos (white arrows) colocalize with endogenous nanos3 accumulations. Nonspecific nuclear aggregates of MCP-GFP (yellow arrowheads) do not colocalize with endogenous nanos3, consistent with these structures being artifacts. Scale bar: 25µm. (K-N) A subset of MCP-GFP accumulations in Cherry-expressing PGCs at 30hpf in cherry-24xMBS-nos3′UTR-injected embryos coincide with Vasa protein-positive germ granules (arrows) whereas others do not (arrowheads). Scale bar: 10µm. The dotted lines denote borders of cells expressing Cherry reporter.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development