ZFIN ID: ZDB-FIG-150505-16
Shimizu et al., 2015 - Mitochondrial Ca(2+) uptake by the voltage-dependent anion channel 2 regulates cardiac rhythmicity. eLIFE   4:253-65 Full text @ Elife
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4

(A) In situ hybridization analysis showed that VDAC2 is expressed in embryonic hearts at 36 hpf (upper image) and 48 hpf (lower image). (B) Injection of 25 pg in vitro synthesized VDAC2 mRNA restored cardiac contractions in 52.9 ± 12.1% (n = 78) of 1-day-old tre embryos, compared to 21.8 ± 5.1% in uninjected siblings (n = 111). (C) Schematic diagram of myl7:VDAC2 construct (top). In situ hybridization analysis showed that TBF treatment induces VDAC2 expression in the heart (lower panel). (D) While only ~20% of myl7:VDAC2;NCX1hMO embryos have coordinated contractions (n = 116), 52.3 ± 2.4% of these embryos established persistent, rhythmic contractions after TBF induction of VDAC2 (n = 154). (E) On average, 71.2 ± 8.8% efsevin treated embryos have coordinated cardiac contractions (n = 131). Morpholino antisense oligonucleotide knockdown of VDAC2 (MOVDAC2) attenuates the ability of efsevin to suppress cardiac fibrillation in tre embryos (45.3 ± 7.4% embryos with coordinated contractions, n = 94). (F) Efsevin treatment restores coordinated cardiac contractions in 76.2 ± 8.7% NCX1MO embryos, only 54.1 ± 3.6% VDAC2zfn/zfn;NCX1MO embryos have coordinated contractions (n = 250). (G) Diagram of Zinc finger target sites. VDAC2zfn/zfn carries a 34 bp deletion in exon 3 which results in a premature stop codon (red asterisk). In situ hybridization analysis showing loss of VDAC2 transcripts in VDAC2zfn/zfn embryos. White arrowheads point to the developing heart.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
vdac2 WT standard conditions Prim-5 to Long-pec anatomical structure ISH
Prim-25 heart tube ISH
Long-pec heart ISH
la2309Tg control Prim-5 to Long-pec heart tube ISH
la2309Tg chemical treatment: pharmaceutical Prim-5 to Long-pec heart tube ISH
vdac2la2256/la2256 standard conditions Prim-5 to Long-pec Not Detected anatomical structure ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la2309Tg + MO2-slc8a1a standard conditions Long-pec heart contraction decreased process quality, abnormal
slc8a1atc318d/tc318d control Long-pec heart contraction decreased process quality, abnormal
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife