Fig. 5

Induced cell death in transition gonads of the dnd:NTR-EGFP transgenic fish via MTZ treatments.

The MTZ-induced cell death were evaluated by an in situ cell death assay in the transition gonads of the dnd:NTR-EGFP transgenic fish at 26 dpf stage after 1 week MTZ treatment. Transgenic fish at 18 dpf were treated with 5 mM MTZ/0.2% DMSO or 0.2% DMSO solution for 1 week. The fish were cryosectioned at 6µm for in situ TUNEL assay. A-D) transition gonad after MTZ treatments with apoptosis signals under fluorescent microscope (red fluorescence, A), merge images under fluorescent microscope and white light microscope (B), DAPI staining (C), HE staining (D); E-H) transition gonad in control groups with apoptosis signals under fluorescent microscope (red fluorescence, E), merge images under fluorescent microscope and white light microscope (F), DAPI staining (G), HE staining (H); The white frame indicates the gonad position.