Fig. S5
Characterization of Motile caRhoA-Expressing Cells In Vivo, Related to Figure 7 (A) Fluorescence time-lapse images of a single polarized stable-bleb cell transplanted from a transgenic Tg(actβ1:lifeact-GFP) donor with mosaic expression of caRhoA plus GPI-RFP into a wild-type host. (B) Localization of Myl12.1-eGFP (myosin II) in a single polarized progenitor cell transplanted from a transgenic Tg(actβ1:myl12.1eGFP) donor with mosaic expression of caRhoA plus GPI-RFP into a wild-type host (left) with kymograph data along cell margin (right). (C) Quantification of Myl12.1-eGFP (myosin II) localization in polarized stable-bleb cells along normalized cell length in vivo (n = 4 cells). (D) Fluorescence time-lapse images of a single polarized stable-bleb cell transplanted from a transgenic Tg(actβ1:myl12.1eGFP) donor with mosaic expression of a constitutively active version of rhoA (caRhoA) plus GPI-RFP plus e-Cadherin morpholinos (MO) into a wild-type host. (E) Exemplary 2D projected in vivo tracks of polarized motile cells expressing either caRhoA (blue) or caRhoA plus e-Cadherin MO (caRhoA+Ecad-MO, red) obtained from transplantation experiments as described in (D). (F) Quantification of average single cell net displacement over time for polarized motile cells expressing either caRhoA (blue) or caRhoA plus e-Cadherin MO (caRhoA+Ecad-MO, red). All scale bars, 20 µm. |
Reprinted from Cell, 160, Ruprecht, V., Wieser, S., Callan-Jones, A., Smutny, M., Morita, H., Sako, K., Barone, V., Ritsch-Marte, M., Sixt, M., Voituriez, R., Heisenberg, C.P., Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility, 673-685, Copyright (2015) with permission from Elsevier. Full text @ Cell