Fig. 4
Paramecia evoke prey capture behavior and a response in AF7. (A) Overlay of 50 frames (167 ms) of high-speed video showing a head fixed larva responding to paramecia. (B) The position of the tip of the tail of the larva in A without paramecia, showing spontaneous swims. (C) The same larva after paramecia were added to the dish. Bouts that were identified as prey capture by the SVM are colored blue. (D) Ath5:Gal4, UAS:GCaMP6s larvae were imaged before and after addition of paramecia. Pseudocolor represents the ratio of the ΔF/F with paramecia to without. (E) AF7 and AF9 responses to paramecia in nine larvae. Maximum ΔF/F is plotted for each trial. The AF7 response was significantly higher in trials with paramecia (p = 9.6 × 105, Wilcoxon rank sum test), whereas the AF9 response was not significantly different (p = 0.083). n = 10 larvae. Error bars = ±SEM. See also Video 2. |