ZFIN ID: ZDB-FIG-150320-17
Lu et al., 2015 - A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation. Developmental Biology   397(2):225-36 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 1 Overexpression of Arl13b increases cilia length in zebrafish embryos in a dose-dependent manner. (A) Primary cilia on somitic cells of wild-type (wt) and Tg(bactin2::Arl13b-gfp) transgenic zebrafish embryos. (B) Quantitation of primary cilia length on somitic cells of wild-type (wt) and Tg(bactin2::Arl13b-gfp) transgenic zebrafish embryos (high GFP expressors). 10 embryos were analyzed from each group, 50 cilia from each embryo. (C) Increase in dosage of Arl13b-gfp mRNA resulted in a proportionate increase in primary cilia length. (D) The protein expression level of different doses of Arl13b-gfp mRNA. (E) Quantitation of primary cilia length increase in response to Arl13b overexpression. 10 embryos were analyzed from each group, 50 cilia from each embryo. Zebrafish embryos were analyzed at 15 hpf. Ciliary axonemes were labeled with anti-acetylated tubulin antibodies (red) and Arl13b was visualized with GFP fluorescence (green). In this and other figures, nuclei were labeled with DAPI (blue), scale bars=1 ┬Ám and error bars represent SEM, unless mentioned otherwise.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP hsc5Tg standard conditions 10-13 somites somite cilium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hsc5Tg standard conditions 10-13 somites somite cilium increased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 397(2), Lu, H., Toh, M.T., Narasimhan, V., Thamilselvam, S.K., Choksi, S.P., Roy, S., A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation, 225-36, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.