|ZFIN ID: ZDB-FIG-150320-17|
Fig. 1 Overexpression of Arl13b increases cilia length in zebrafish embryos in a dose-dependent manner. (A) Primary cilia on somitic cells of wild-type (wt) and Tg(bactin2::Arl13b-gfp) transgenic zebrafish embryos. (B) Quantitation of primary cilia length on somitic cells of wild-type (wt) and Tg(bactin2::Arl13b-gfp) transgenic zebrafish embryos (high GFP expressors). 10 embryos were analyzed from each group, 50 cilia from each embryo. (C) Increase in dosage of Arl13b-gfp mRNA resulted in a proportionate increase in primary cilia length. (D) The protein expression level of different doses of Arl13b-gfp mRNA. (E) Quantitation of primary cilia length increase in response to Arl13b overexpression. 10 embryos were analyzed from each group, 50 cilia from each embryo. Zebrafish embryos were analyzed at 15 hpf. Ciliary axonemes were labeled with anti-acetylated tubulin antibodies (red) and Arl13b was visualized with GFP fluorescence (green). In this and other figures, nuclei were labeled with DAPI (blue), scale bars=1 µm and error bars represent SEM, unless mentioned otherwise.
|GFP||hsc5Tg||standard conditions||10-13 somites||IHC|
|hsc5Tg||standard conditions||10-13 somites||somite cilium increased length, abnormal|
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Reprinted from Developmental Biology, 397(2), Lu, H., Toh, M.T., Narasimhan, V., Thamilselvam, S.K., Choksi, S.P., Roy, S., A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation, 225-36, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.