Fig. 3

pard3 is not essential for neural crest migration. A,B: Representative images of cranial neural crest marked by Tg(sox10:memRFP) reporter expression in 30 hpf control pard3^fh305/+ (A) and MZpard3^fh305 mutant (B) embryos. View is from lateral with anterior to the left and dorsal up. Arrows indicate migrating groups of neural crest cells. In some regions, indicated by the outlined boxes and magnified in the insets, neural crest cells appear more tightly packed in mutant than in control. ov, otic vesicle. C,D: Representative images of jaw structures at 5 dpf in wild-type (C) and MZpard3^fh305 mutant (B) larvae. The jaw in the mutant larva appears similar to that of wild-type. E,F: Representative frames captured from time-lapse movies of trunk neural crest marked by Tg(sox10:memRFP) reporter expression. Motor neurons and axons are marked by Tg(mnx1:GFP) expression (green) in the final frames of the sequences. Images are from lateral with anterior to the left and dorsal up. Numbers indicate time, in minutes, elapsed from beginning of imaging. Images were captured from 18–24 hpf. In the control embryo (E), neural crest cells form streams (asterisks) with little overlap at points of contact (arrows). By contrast, neural crest cells appear less organized and overlap more in the MZpard3^fh305 mutant embryo (F). Scale bar = 100 µM in E,F.