FIGURE

Fig. 5

ID

ZDB-FIG-150226-3

Publication

Pan et al., 2014 - Structural and temporal requirements of Wnt/PCP protein Vangl2 function for convergence and extension movements and facial branchiomotor neuron migration in zebrafish

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Fig. 5

Vangl2 functions during the early stages of FBM neuron migration. Summary of effects on FBM neuron migration following heat shock-induced Vangl2 transgene expression. Embryos were heat shocked at various times (10, 12, 15, 18, 21, 24 and 27 hpf), and migration phenotypes were scored at 48 hpf. Expression of Vangl2 FL (A) and Vangl2 TM-C (B) generated similar effects. Heat shock between 10 and 15 hpf resulted in migration failure in >80% of embryos. By contrast, heat shock at 18 hpf or later resulted in few or no embryos with non-migrated neurons. However, most of these embryos exhibited varying degrees of migration block in r5 (see Fig. 4F and H). Number of embryos scored in parenthesis for each construct. Data pooled from 2 to 5 experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg; vu22Tg; zou003Tg rw0Tg; vu22Tg; zou012Tg
Condition:	heat shock
Observed In:	branchiomotor neuron neuron migration
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 131, Pan, X., Sittaramane, V., Gurung, S., and Chandrasekhar, A., Structural and temporal requirements of Wnt/PCP protein Vangl2 function for convergence and extension movements and facial branchiomotor neuron migration in zebrafish, 1-14, Copyright (2014) with permission from Elsevier. Full text @ Mech. Dev.