Fig. 5

Lateralised habenular neurogenesis is compromised by treatments that disrupt Nodal signalling. Plots of the numbers of HuC+ neurons in the left and right habenulae and corresponding asymmetry indices (AI) in ntl morphants (A,B), spaw morphants (C,D) and cold-treated zebrafish embryos (E,F). (A,C,E) Habenular neurons were detected in Tg(huC:gfp) transgenic embryos using either anti-GFP (A,E) or HuC/D (C) antibody. A symmetry line (black) is included as a reference. Dotted lines represent the best-fit linear regression for each experimental condition. (B,D,F) AI values plotted for all embryos. The same control dataset is plotted for the ntl MO and cold-treatment experiments. Applying the Bonferroni correction for multiple comparisons shows that both the cold-treated and ntl MO datasets are significantly different from the control at the 5% confidence level. Horizontal black lines indicate the median AI value for the group of like-treated embryos.