Fig. 6

pard3 and prkci Are Functionally Relevant miR-219 Targets

(A–C) Lateral images of living 3 dpf (Tg:olig2:EGFP) larvae, focused on the trunk spinal cord. Control larva (A) shows the normal number and distribution of dorsally migrating OPCs (arrow). Whereas miR-219 MO-injected larvae have few OPCs (B), larvae coinjected with miR-219 and pard3 MOs have an intermediate number of OPCs (C).

(D) Graph showing quantification of OPC phenotypic classes. Larvae classified as normal had the number of dorsally migrated OPCs typical of wild-type. Larvae were classified as severe if fewer than five OPCs had migrated and mild in all other circumstances. p value was calculated by comparing the number of larvae with normal numbers of OPCs in the miR-219-MO alone and miR-219 MO ± pard3 MO experiments. Data represent ± SEM (n = 25 larvae per group, with three replicates). p = 0.0324, unpaired t test.

(E and F) Larvae injected with pard3 TP MO have fewer OPCs than those injected with a control TP MO.

(G) Graph showing quantification of the pard3 TP MO phenotypes. Data represent ± SEM (n = 35 and 55 larvae in two independent experiments). p < 0.0001, unpaired t test.

(H and I) Larvae injected with prkci TP MO have fewer OPCs than those injected with a control TP MO.

(J) Graph showing quantification of the prkci TP MO phenotype. (n = 45–60 larvae per experiment, with three independent experiments). p = 0.0020, unpaired t test.