FIGURE

Fig. 6

ID

ZDB-FIG-140903-7

Publication

Krauss et al., 2014 - Endothelin signalling in iridophore development and stripe pattern formation of zebrafish

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Fig. 6

Morpholino-mediated knockdown of edn3b function in wild-type and alb embryos results in a strong reduction of iridophores.

(A–A3) Morpholinos designed to interfere with splicing or translation of edn3b were injected into alb embryos, RNA in situ hybridization for pnp4a at 48 hpf shows a reduction of iridophore numbers in the dorsal stripes (marked with asterisks in panels A and A2). At 5 dpf morpholino injected larvae show strong reductions in iridophore numbers. Injection of a 5-bp-mismatch control morpholino had no effect (B). RT-PCR results to detect edn3b spliced message (C). Whereas the mismatch control morpholino showed no effect on the level of edn3b transcript, the PCR did not result in amplification of edn3b message after injection of the splice-interfering morpholino.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc45a2^b4/b4 + MO1-edn3b slc45a2^b4/b4 + MO2-edn3b
Knockdown Reagents:	MO1-edn3b MO2-edn3b
Observed In:	dorsal larval melanophore stripe iridophore
Stage:	Day 5

Phenotype Detail

Acknowledgments

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