FIGURE

Fig. 2

ID

ZDB-FIG-140807-10

Publication

Kurtenbach et al., 2013 - Pannexin1 channel proteins in the zebrafish retina have shared and unique properties

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

(A) qRT-PCR analysis of drPanx1 expression. Total RNA was isolated from several CNS regions of adult zebrafish und used for cDNA synthesis. Retinal drPanx1 expression was normalized to 1. Each bar represents the mean + SEM. (n = 3) (B-D) IHC/DAB staining of drPanx1b expression in the retina. The anti-drPanx1b antibody was used as primary antibody. Cell bodies were stained with propidium iodide (red; B) or cresyl violet (violet; C, D). Arrows point at drPanx1b positive cells in the INL and GCL. (B) The primary antibody was visualized by an anti-chicken antibody conjugated with Alexa488. (C, D) For DAB staining, a biotinylated anti-chicken antibody served as detection antibody. (D) Control staining using the immunizing blocking peptide together with the anti-drPanx1b antibody. (Scale bar (B-D) = 10 µm; GCL = ganglion cell layer; INL = inner nuclear layer; IPL = inner plexiform layer; ONL = outer nuclear layer; OPL = outer plexiform layer; PR = photoreceptors).

Expression Data

Genes:	panx1a panx1b
Antibody:	Ab1-panx1b
Fish:	WT
Anatomical Terms:	brainstem cerebellum olfactory bulb optic tectum retina retinal ganglion cell layer retinal inner nuclear layer spinal cord tegmentum
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One