FIGURE

Fig. 1

ID

ZDB-FIG-140728-15

Publication

Acosta et al., 2014 - Mutant Human FUS Is Ubiquitously Mislocalized and Generates Persistent Stress Granules in Primary Cultured Transgenic Zebrafish Cells

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Fig. 1

Whole mount and cell cultures of FUS-GFP transgenic zebrafish.

(A) Transgenic zebrafish larvae whole mounts showed cytosolic mislocalization of mutant human FUS in FUS-R521C-GFP in comparison to FUS-WT-GFP which was restricted to cell nuclei. (B) FUS-R521C-GFP showed greater cytosolic distribution in comparison to FUS-WT-GFP in zebrafish primary cell cultures. (C) Confocal images of 48 hpf transgenic zebrafish spinal cord further demonstrate mislocalization of mutant FUS-521C-GFP (green) in motor neurons (red) (arrows). Images are maximum projections captured using a Leica SPE5 confocal microscope. Sagittal sections (upper images) of Tg(s1020tGAL4: UASmCherry) (Scott and Baier, 2009) and transverse sections (lower images) of Tg(HB9: mK02caax) membrane localised mk02 expressed in motorneurons by HB9 promoter (Flanagan-Steet et al 2005) with either FUS-WT-GFP or FUS-521C-GFP as indicated. Scale bar = 20 μm.

Expression Data

Genes:	GFP mCherry mKOFP2
Fish:	mq1Tg mq2Tg mq1Tg; mq7Tg mq1Tg; s1020tEt; s1984tTg mq2Tg; mq7Tg mq2Tg; s1020tEt; s1984tTg
Condition:	primary cell culture
Anatomical Terms:	motor neuron motor neuron cytosol spinal cord whole organism cytosol whole organism nucleus
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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