FIGURE

Fig. S6

ID

ZDB-FIG-140710-19

Publication

Walsh et al., 2011 - Planar polarity pathway and Nance-Horan syndrome-like 1b have essential cell-autonomous functions in neuronal migration

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Fig. S6

nhsl1b^fh131 mutant facial branchiomotor (FBM) neurons require wild-type FBM neurons for migration. (A-D) Confocal images of fixed mosaic embryos at 48 hours post-fertilization (hpf) with anterior to the top. Lysinated rhodamine dextran marks donor-derived cells (red); anti-Isl1 antibody marks all cranial motor neurons (donor and host-derived, blue); and Tg(isl1:GFP) marks donor-derived motor neurons (green). Histograms on the right indicate the percent of donor-derived FBM neurons in rhombomere (r)4 (unmigrated), r5 and r6 (fully migrated) under the transplantation conditions indicated on the far left, which are written as Donor>Host. n refers to the total number of FBM neurons scored in each condition. hoxb1a morpholinos were used in B and D to prevent host FBM neurons from migrating by a cell-autonomous mechanism. Scale bar: 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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