FIGURE

Fig. 2

ID

ZDB-FIG-140523-64

Publication

Nishiya et al., 2014 - A Zebrafish Chemical Suppressor Screening Identifies Small Molecule Inhibitors of the Wnt/beta-catenin Pathway

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Fig. 2

A Zebrafish Chemical Screening Identifies Inhibitors against GGTase, Akt, Telomerase, and K⁺ Channel as Chemical Suppressors of the Wnt/ß-catenin Pathway

(A) Compounds from SCADS chemical libraries were classified into subcategories of the target molecules.

(B) The 20 µm GGTI-286, Akt inhibitor IV (AKTiIV), dequalinium, and ß-rubromycin rescued the BIO-induced eyeless phenotype. Images were taken at 30 hpf.

(C) GGTI-286, AKTiIV, dequalinium, and ß-rubromycin reduced ß-catenin/TCF-dependent transcriptional activity in CHO cells. Cells were transiently cotransfected with Super 8 x TOPFlash for monitoring ß-catenin/TCF-dependent transcriptional activity and with pRL-CMV for normalizing transfection efficiency. The cells were pretreated with 10µm compounds for 30 min to 6 hr and treated with 2µm BIO for 18 hr. Cells were lysed, assayed for firefly luciferase activity, and then assayed for Renilla luciferase activity. Normalized relative luciferase activities are shown as fold activation to DMSO-treated cells. Values are ± SEM (n = 3). AU, arbitrary units.

(D) Structures and known target molecules of the positive compounds are summarized.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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