Fig. 4
- ID
- ZDB-FIG-140522-53
- Publication
- Ouchi et al., 2014 - The heterochronic genes lin-28a and lin-28b play an essential and evolutionarily conserved role in early zebrafish development
- Other Figures
- All Figure Page
- Back to All Figure Page
Effect of lin-28a and lin-28b knockdown on cell proliferation. A. Confocal images of BrdU (Red) incorporation in lin-28a and lin-28b morphant embryos at 5hpf. Both lin-28a and lin-28b MO-injected embryos were exposed to BrdU at 4 hpf for 1 hour. The nuclei were stained with DAPI (blue). B. Percentage of BrdU incorporated cells in lin-28a and lin-28b morphants embryos. The percentage of BrdU-incorporated cells was calculated by dividing the number of BrdU-positive cells by the total number of cells as determined by DAPI-stained cells. The percentage of BrdU-positive cells was significantly lower in both lin-28a MO- and lin-28b MO-injected embryos compared with control MO-injected embryos (***p<0.001, control MO vs. lin-28a MO, control MO vs. lin-28b MO, two-tailed test, n = 10, mean ± SEM). |
Genes: | |
---|---|
Fish: | |
Knockdown Reagents: | |
Anatomical Terms: | |
Stage Range: | 30%-epiboly to Prim-5 |
Fish: | |
---|---|
Knockdown Reagents: | |
Observed In: | |
Stage: | 30%-epiboly |