Fig. 1

The clbn phenotype is characterized by abnormalities in the digestive organs (A and B) Brightfield images of left lateral views and (C) histology of WT and clbn^s846 larvae at 120 hpf show a thick, folded intestinal epithelium (black arrows in C) and inflated swim bladder in WT, whereas clbn displays a thin, unfolded intestinal epithelium, delayed yolk resorption, and smaller eyes. (D) Two-photon microscopy of WT and clbn^s846 larvae on the Tg(gutGFP)^s854 background and (E) epifluorescence microscopy on the Tg(fabp10:RFP,ela3l:EGFP)^gz12 background reveal smaller digestive organs in clbn^s846 compared with WT. The two images in A were taken at the same magnification, as were the two images in each of panels B, D, and E. The clbn phenotype is 100% penetrant and all larvae die between 7 and 10 dpf; however, the severity of the morphological abnormalities may vary between clutches. For example, the digestive organ phenotype is visibly conspicuous in some clutches at around 108 hpf and not in others until 120 hpf. a, anus; e, eye; es, esophagus; ib, intestinal bulb; Li, liver; p, pancreas; sb, swim bladder; y, yolk. (Scale bar in C, 100 µm.)