FIGURE

Fig. 3

ID

ZDB-FIG-140507-17

Publication

Ogryzko et al., 2014 - Zebrafish tissue injury causes up-regulation of interleukin-1 and caspase dependent amplification of the inflammatory response

Other Figures

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Fig. 3

Caspase-1 inhibitor YVAD downregulates NF-κB activation in response to injury. (A) Fluorescence photomicrograph of pNF-κB:EGFP embryos following tailfin transection at 1 hpi (i) and 8 hpi (ii) indicating the region quantified (square bracket). Scale bars: 100 μm. (B) There is a 2.4-fold increase in EGFP fluorescence in response to injury, quantifiable as average fluorescent intensity across the transection site (indicated in A). ****Pd0.0001 by t-test. (C) Embryos treated with 50 μM YVAD show a reduction in EGFP fluorescence at 8 hpi compared with DMSO-treated controls and embryos treated with qVD. **Pd0.01 by one-way ANOVA with Dunnett’s post-test. n=30 performed as three independent experiments.

Expression Data

Gene:	EGFP
Fish:	sh235Tg
Conditions:	transection: caudal fin chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor, transection: caudal fin
Anatomical Term:	caudal fin
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sh235Tg
Conditions:	transection: caudal fin chemical treatment by environment: EC 3.4.22.36 (caspase-1) inhibitor, transection: caudal fin
Observed In:	caudal fin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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