Fig. 4
- ID
- ZDB-FIG-140421-4
- Publication
- Wilkins et al., 2014 - A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver
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Generation of transgenic zebrafish for liver translating ribosome affinity purification (TRAP). (A–C) Confocal projection of dissected liver and gallbladder from a 5 dpf Tg(krt18:TRAP) larva, showing EGFP-Rpl10a fusion protein (A) and biliary marker 2F11 (B), which co-localize (C; EGFP – green; 2F11 – red). EGFP is also visualized in krt18-expressing cells of the esophagus (e) and skin (s). (D) High-magnification confocal scan of liver from the same larva shows localization of TRAP fusion protein to biliary cell cytoplasm and nucleus, with a subset showing nucleolar enrichment (insets). (E–G) Confocal projection of dissected liver and gallbladder from 5 dpf Tg(lfabp:TRAP) larva, showing hepatocyte expression of TRAP fusion protein (E) that is non-overlapping with biliary 2F11 staining (F). (H) High-magnification confocal scan of liver from same larva, showing localization of TRAP fusion protein to the hepatocyte cytoplasm and nucleolus (inset), exclusive of biliary cells stained red with the 2F11 antibody. |
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| Stage: | Day 5 |
Reprinted from Gene expression patterns : GEP, 14(2), Wilkins, B.J., Gong, W., and Pack, M., A novel keratin18 promoter that drives reporter gene expression in the intrahepatic and extrahepatic biliary system allows isolation of cell-type specific transcripts from zebrafish liver, 62-68, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns