Fig. 2

Histopathology of edn1 transgenic hepatocytes.

(A1–A6) H&E staining of the liver sections from the GFP-mCherry control fish at 11 months (A1), and from the edn1 transgenic fish at 3, 5, 7, 9 months (A2–A6). The images were captured at 200X magnification. The A12–A62 images resemble the A1–A6 images, though they were captured at 400X magnification. H&E staining of liver sections from GFP-mCherry control fish showed normal histology, while edn1 transgenic fish showed normal, steatosis, bile duct dilation, hyperplasia, and HCC at 3, 5, 7, and 9 months. Scale bars: 50 μm. (B). The Tg(fabp10a:GFP-mCherry) and Tg(fabp10a:edn1-pA/CG2) liver sections were stained by H&E (B1 and B2). The same sections were also stained with oil red O (B3 and B4). Tissues from the Tg(fabp10a:edn1-pA/CG2) fish showed positive staining with oil red O in the hepatocytes (400X). Scale bars: 50 µm. (C) The cumulative frequency of steatosis, bile duct dilation, hyperplasia, and HCC in the edn1 transgenic fish was determined by Kaplan-Meier analysis. The different colors denote different liver diseases: steatosis (green), bile duct dilation (blue), hyperplasia (brown), and HCC (red). The differences between the edn1 transgenic fish and control fish were assessed using a two-tailed Student′s t-test. The P value is less than 0.05.