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ZFIN ID: ZDB-FIG-140324-22
Samson et al., 2013 - 3-OST-7 regulates BMP-dependent cardiac contraction. PLoS Biology   11(12):e1001727 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 1

3-OST-7 is required for ventricular contraction in the zebrafish heart.

Lateral views of control (uninjected, wild-type) (A) and 3-OST-7 morphant (B) embryos at 48 hpf showing edema (red arrowhead) and blood pooling (black arrowhead) with knockdown of 3-OST-7. Lateral views of hearts in control (C–D) and 3-OST-7 morphant (F–G) Tg(cmlc2:gfp) embryos at periods of ventricular diastole (C and F) and systole (D and G). 3D-reconstructed ventricular cell in control (E) and 3-OST-7 morphant (H) embryos show decreased volume (V) in morphants. (I) Gene structure of 3-OST-7 showing MO targets (red for MO1 and green for MO2) and primer sets used for RT-PCR analysis (blue and orange bars). RT-PCR analysis showing decrease in spliced and increase in unspliced products with increasing dose of MO injected. (J) Percentage contraction of embryos in different 3-OST knockdowns (3-OST-7, 3-OST-5, 3-OST-3Z). Only knockdown of 3-OST-7 MO resulted in ventricular noncontraction. (K) Percentage contraction of embryos in rescue experiments using three different transgenes. Uninjected embryos with (green bar) or without (white bar) the transgene had normal ventricular contraction. Injection of 3-OST-7 MO2 in Tg(β-actin:3-OST-7-IEP) and Tg(fli1:3-OST-7-IEP) embryos rescued the noncontracting ventricle phenotype (p = 0.039 and p = 0.0036, respectively, *). Injection of 3-OST-7 MO2 in Tg(cmlc2:3-OST-7-IEP) did not rescue the phenotype (p = 0.44, NS). Graphs depict the percentage of embryos with phenotype in 3-OST-7-overexpressing (GFP+, green hatched) or non-3-OST-7-overexpressing (GFP-, white hatched) embryos from individual crosses between one of three transgenic founders and wild-type AB zebrafish. At, atrium; V, ventricle; error bars, SEM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Long-pec heart IFL
twu34Tg + MO2-hs3st1l1 standard conditions Long-pec heart IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-hs3st1l1 standard conditions Long-pec blood accumulation pericardium, abnormal
Long-pec pericardium edematous, abnormal
twu34Tg + MO2-hs3st1l1 standard conditions Long-pec blood circulation decreased process quality, abnormal
Long-pec cardiac ventricle hypoplastic, abnormal
Long-pec cardiac ventricle cardiac muscle cell decreased volume, abnormal
Long-pec cardiac ventricle heart contraction decreased process quality, abnormal
Long-pec cardiac ventricle heart contraction process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.