FIGURE

Fig. 5

ID

ZDB-FIG-140228-29

Publication

Sagerström et al., 1996 - Anteroposterior patterning in the zebrafish, Danio rerio: an explant assay reveals inductive and suppressive cell interactions

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Fig. 5

Ventral marginal zone explants suppress the expression of otx2 in animal caps. (A) Schematic representation of dissections and conjugate preparation. Animal caps from sphere stage embryos (late blastula, 4 hpf) were aggregated into groups of 10 and either cultured alone or conjugated to ventral marginal zone (VMZ) explants from shield stage embryos (early gastrula, 6 hpf). Each conjugate contained 5 animal caps and 1 VMZ explant. Animal caps and conjugates were harvested when control embryos reached the 12 somite stage (15 hpf, 9 hours of culture). D, dorsal; V,ventral. (B) Whole-mount in situ hybridization analysis of otx2 expression. Animal caps (a) and conjugates (b) were analyzed for otx2 expression after 9 hours in culture. The VMZ lineage label (see Materials and Methods) is visualized in light blue and animal caps are unlabelled. This data is included in Table 3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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