Fig. 4

Higher Proportion of RGCs with Diffuse Dendritic Arbors in teneurin-3 Morphants

(A) Lateral view of mosaically labeled RGCs in the retina of a 5 dpf tenm3 MO-injected larva. Scale bar, 20 μm. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer.

(B) Bar graph showing the proportions of 5 dpf RGCs possessing monostratified (cyan, C), bistratified (green, D), multistratified (yellow, E), and diffuse (magenta, F) dendritic arbors relative to the total number mosaically labeled RGCs within each animal group (WT n = 89 cells in 34 larvae; control MO n = 92 cells in 39 larvae; tenm3 MO n = 98 cells in 49 larvae).

(C–F) Representative RGCs with monostratified (C), bistratified (D), multistratified (E), and diffuse (F) dendritic arbors. All images represent maximum intensity projections of ~30 μm confocal z stacks that have been pseudocolored and rotated to best show dendritic arborizations. Scale bars, 20 μm.

(G) Summary table showing the morphological classification and frequency of the 11 RGC types within each group (number of cells found per each type are reported in brackets). In tenm3 morphants, four diffuse RGCs (4.1% of cells) showed dendritic arborization patterns that could not be classified in any of the 11 types and, hence, were not included in the table.