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ZFIN ID: ZDB-FIG-140124-14
Matsuda et al., 2013 - Aldh1-expressing endocrine progenitor cells regulate secondary islet formation in larval zebrafish pancreas. PLoS One   8(9):e74350 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Days 14-20 to Days 30-44

Fig. 3

Aldh1-expressing cells express the pancreatic epithelial marker 2F11.

(A-D) All Aldh1-expressing cells co-express the pancreatic epithelial marker 2F11 (white arrows). (E) 2F11 does not label GRCFPpos vascular endothelial cells in Kdrl:GRCFP fish. (F-H) 2F11 labels both a subset of Insulinpos cells (G and G′) and eGFPpos cells in NeuroD:eGFP (F and F′), Pax6b:eGFP (G and G′) Hb9:eGFP (H) fish. White arrows in A-D indicate cells co-labeling for both 2F11 and Aldh1. White arrows in F and F′ indicate Insulin-negative cells co-expressing 2F11, Aldh1 and NeuroD:eGFP. White arrows in G and G′ indicate Aldh1-negative cell co-expressing Insulin, 2F11 and Pax6:eGFP. White arrow in H indicates Aldh1-negative cell expressing Insulin, 2F11 and Hb9:eGFP; blue arrows indicate Insulin-negative, Hb9:eGFP-negative cells expressing 2F11 and Aldh1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP jh1Tg standard conditions Days 14-20 exocrine pancreas IHC
Days 21-29 exocrine pancreas IHC
Days 30-44 exocrine pancreas IHC
nl1Tg standard conditions Days 14-20 pancreas endocrine cell IHC
GFP ml5Tg standard conditions Days 21-29 pancreatic B cell IHC
ulg515Tg standard conditions Days 14-20 pancreas endocrine cell IHC
grcfp zn1Tg standard conditions Days 14-20 vascular endothelium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-2F11 IHC jh1Tg standard conditions Days 14-20 pancreatic duct epithelium
IHC Days 21-29 pancreatic duct epithelium
IHC Days 30-44 pancreatic duct epithelium
IHC ml5Tg standard conditions Days 21-29 pancreatic duct epithelium
IHC nl1Tg standard conditions Days 14-20 pancreatic duct epithelium
IHC ulg515Tg standard conditions Days 14-20 pancreatic duct epithelium
IHC zn1Tg standard conditions Days 14-20 pancreatic duct epithelium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One