FIGURE

Fig. S7

ID

ZDB-FIG-140115-29

Publication

Kim et al., 2013 - A Complex of BBS1 and NPHP7 Is Required for Cilia Motility in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

Dextran injection revealed fluid excretion via the cloaca in zbbs1 and znphp7.2 morphants. Zebrafish control and morphant embryos with pronephric cysts at 96 hpf were injected with 5% FITC-conjugated dextran solution (70 kD) into the circulation. Fluorescent dye excretion with the urine at the cloaca (black and white arrows) was observed in control embryos (22/22), zbbs1 (16/16) and znphp7.2 (21/23) morphants. The lower panel represents a znphp7.2 morphant embryo with missing fluorescent dye excretion due to persistent closure of the cloaca (arrowheads). Images on the left column represent transmitted light images and images on the right column represent fluorescent images of the same embryo for each setting.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-glis2b
Knockdown Reagent:	MO1-glis2b
Observed In:	cloaca excretion
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One