FIGURE

Fig. 4

ID

ZDB-FIG-131220-30

Publication

Yasuda et al., 2013 - A cis-acting element in the coding region of cyclin B1 mRNA couples subcellular localization to translational timing

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Fig. 4

Localization of cyclin B1 reporter mRNAs in the animal polar cytoplasm of transgenic zebrafish oocytes. (A) Section in situ hybridization of a full-grown oocyte probed with cyclin B1. The cyclin B1 mRNAs were localized to the animal polar cytoplasm of the oocyte beneath the micro-pile as an aggregation (arrow). ((B)?(H)) Section in situ hybridization probed with gfp, showing full-grown oocytes of transgenic zebrafish expressing tgo3′ (B), 1?523 (C), 524?1197 (D), 524?736 (E), 737?949 (F), 949?1197 (G) and tgo^M3′ (H) mRNAs. The tgo3′, 524?1197 and 524?736 mRNAs were aggregated beneath the micro-pile (arrows) ((B), (D), (E)), whereas the 1?523, 737?949, 949?1197 and tgo^M3′ mRNAs had no signal ((C), (F), (G), (H)). Arrowheads indicate the micro-pile. Dotted lines encircle aggregated mRNAs. Bars, 100 μm.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Biology, 382(2), Yasuda, K., Kotani, T., and Yamashita, M., A cis-acting element in the coding region of cyclin B1 mRNA couples subcellular localization to translational timing, 517-29, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.