FIGURE

Fig. 2

ID

ZDB-FIG-131108-25

Publication

Gratacap et al., 2013 - Mucosal candidiasis elicits NF-κB activation, proinflammatory gene expression and localized neutrophilia in zebrafish

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Fig. 2

NF-κB is highly activated in epithelial cells in vivo in high-level infection. (A?E) Cohorts of twenty NFκB:EGFP fish were infected by immersion with C. albicans CAF2-dTomato and imaged by confocal microscopy at 5 dpi. (A?C) In vivo expression of GFP in high-level-infected (A) and uninfected (B) swimbladder; yellow arrows indicate the epithelial layer. (C) Magnification of image A (white box), with C. albicans inside a phagocyte (white arrows). (D,E) Ex vivo dissected swimbladder expression of EGFP from high-level infected zebrafish (D) with highlighted epithelial (green arrow towards green box) and mesothelial (black arrow towards gray box) layers and uninfected swimbladder (E). Scale bars: 100 μm (A,B,D,E) and 20 μm (C). Maximum projections of n slices: n=8 for A and B, n=1 for C?E. Images are representative of four independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nc1Tg (AB)
Condition:	fungal treatment by exposure to environment: Candida albicans
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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