FIGURE

Fig. S2

ID

ZDB-FIG-131105-6

Publication

Wada et al., 2013 - Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Effect of MOs on Splicing of the Gene Transcripts, Related to Figure 2

(A) MOs were targeted at the first exon - second intron junction to block splicing. In all cases, a premature stop codon which abolishes protein function was predicted from the genomic DNA sequences. RT-PCR analyses were performed to detect the transcripts using the primer sets indicated by arrows.
(B) Gel images for RT-PCR. The predicted size of PCR products was dkk1a: 291 bp, dkk1b: 276 bp, dkk2: 261 bp, and krm: 363 bp, respectively (shown by arrowheads). Amounts of the RT-PCR products were significantly reduced from the MO-injected embryos.
(C) Specificity of MOs was tested by injecting corresponding control MOs with five mispaired residues (5mis). Mean ± SEM are indicated. *P < 0.001 (t test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.