FIGURE

Fig. S8

ID

ZDB-FIG-131029-31

Publication

Powell et al., 2013 - Prdm1a directly activates foxd3 and tfap2a during zebrafish neural crest specification

Other Figures

All Figure Page

Back to All Figure Page

Fig. S8

prdm1a dominant activator rescues foxd3 and tfap2a in prdm1a mutant embryos, and both activator and repressor forms rescue sox10. (A-H) Whole-mount ISH was performed on prdm1a^–/– embryos injected with prdm1aDBD-VP16 activator or prdm1a DBD-EnR repressor at 2-somites for foxd3 (A-D, dorsal views) and tfap2a (E-H, lateral views). foxd3 and tfap2a are both decreased in prdm1a^–/– embryos (B,F) compared with WT/het embryos (A,E). Injection of prdm1aDBD-VP16 partially rescues foxd3 (C) and expands tfap2a (G) at the NPB in prdm1a mutants, whereas prdm1aDBD-EnR does not rescue either foxd3 or tfap2a mRNA expression. (I-M) ISH for sox10 was performed on prdm1a^–/– embryos injected with prdm1aDBD-VP16, prdm1aDBD-EnR or both combined at 4-somites. prdm1aDBD-VP16 (K), prdm1aDBD-EnR (L) and both combined (M) partially rescued sox10 expression in the NPB. Dorsal views.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development